Falsely low urinary albumin concentrations after prolonged frozen storage of urine samples.
نویسندگان
چکیده
Prolonged Frozen Storage of Urine Samples, Jacoline W. Brinkman, Dick de Zeeuw, Jacko J. Duker, Ronald T. Gansevoort, Ido P. Kema, Hans L. Hillege, Paul E. de Jong, and Stephan J.L. Bakker (Departments of 1 Clinical Pharmacology, 2 Internal Medicine, and 3 Pathology and Laboratory Medicine, and the 4 Trial and Coordination Center, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands; * address correspondence to this author at: University of Groningen and University Medical Center Groningen, Department of Internal Medicine, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands; fax 31-50-3639069, e-mail [email protected])
منابع مشابه
Apparent loss of urinary albumin during long-term frozen storage: HPLC vs immunonephelometry.
BACKGROUND Urinary albumin detection by immunonephelometry is decreased by approximately 30% in samples that have been frozen at -20 degrees C. An HPLC method for assessment of urinary albumin that detects immunoreactive and immunochemically nonreactive albumin has been introduced as an alternative to immunonephelometry. We investigated whether this technique is affected by sample temperature, ...
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Accurate measurement of albumin excretion rates is important in choosing treatment regimens that may reverse early diabetic renal damage. We report here determinations of slight albuminuria ("microalbuminuria") by radioimmunoassay of fresh specimens, frozen aliquots (stored at -20 degrees C for two, eight, and 24 weeks), and refrigerated specimens (stored at 4 degrees C for one, two, and eight ...
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BACKGROUND AND OBJECTIVES This study investigated the effect of long-term storage at -70 degrees C on urinary albumin, protein, and creatinine measurements in the Family Investigation of Nephropathy and Diabetes, a multicenter study designed to identify genes for diabetic nephropathy. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Spot urine samples were collected at eight centers and shipped ...
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Creatine kinase (CK; EC 2.7.3.2) has been used as an indicator of myocardial cellular damage. In this study we used a Krebs-Henseleit (KH) solution to reperfuse isolated rat hearts after 24 h of cold preservation and collected the KH reperfusate for assay of CK to assess cellular damage. We wanted to determine the stability of CK in the KH solution at different cold-storage temperatures and alb...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 51 11 شماره
صفحات -
تاریخ انتشار 2005